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KMID : 1161420120150080733
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Journal of Medicinal Food
2012 Volume.15 No. 8 p.733 ~ p.740
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Myricetin Protects Against Cytokine-Induced Cell Death in RIN-m5f β Cells
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Ding Ye
Zhang Zhao-Feng
Dai Xiao-Qian
Li Yong
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Abstract
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Cytokine-induced cell death is recognized as a major cause of progressive β-cell loss. Tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and interferon γ (IFN-γ) in combination trigger a series of events that lead to β-cell death. In the past few decades, the use of myricetin as an anti-inflammatory and cytoprotective agent has gained much attention. The present study focused on the protective roles of myricetin against cytokine-induced cell death in insulin-secreting RIN-m5f β cells. The results showed that myricetin (especially at concentrations of 10?μM and 20?μM) increased cell viability and decreased cell apoptosis induced by the cytokine mixture of TNF-α (10?ng/mL), IL-1β (5?ng/mL), and IFN-γ (1000?IU/mL) for 3 days. Moreover, the cytokines increased the total and p65 subunit levels of nuclear factor κB, decreased inhibitor κB α levels, stimulated the accumulation of nitric oxide, increased cytochrome c release from mitochondria, and induced reactive oxygen species generation; myricetin (especially at the concentration of 20?μM) abolished all of these parameters. These results suggest that myricetin might have therapeutic value for preventing β-cell death.
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KEYWORD
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cytokines, mitochondrial death pathway, myricetin, nuclear factor κB, RIN-m5f β cells
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